Gradient preparation
Treatment with collagenase and centrifugation
Repository version control in R studio
Procedure to find the best parameters to segment intracellular particles with the SiCE spot detector macro
Larval body wall dissection at 3-fold time-lapse.
Example of a BALB/c mice performing reversal
Stereotaxic surgery.
Isolation of striatum from embryonic E16 mouse brain
Procedure to install the Sice Spotdetector Macro and related Plugins
Injection of flies.
Image processing of the reference image to correct for uneven illumination
Recording of the locomotor behavior of the flies
Mincing of adipose tissue
Separate the common carotid artery from the Vagus and aortic depressor nerve
Mouse dissection
Intradermal inoculation of the mouse ear
Example video of the experimental setup and analysis.
Separation and sorting of the ovarioles
Separation of spores from sporangia
Calibration of the force applied to a 4.5 μm bead.
Short tutorial for membrane-based 3D image segmentation using Imaris Cell.
Merging of all the acquired files together to create a linearized image of HCs using main1.m
Observational Parameters–Central Nervous System
Movie showing how to measure mesocotyl lengths using FIJI
Steps to use the Kinetic Evaluation Software
Sample video of an entire play behavior test
Assembly and use of the setup
Imaging of inner HCs using the main2.m program
Zebrafish euthanasia and harvesting of the eye
Flux vs. time
Measurement of Fv/Fm
Video-EEG of a mouse exhibiting SE
Imaging of outer HCs using the main3.m program
Fluorescence intensity measurement
Assembly of the transfer pack in a semi-dry blotter
Gel electroelution protocol.
Demonstration of the T-maze construction
Tracking of microglial processes.
Visualization of the protocol as a video recording of the procedures
Example video of a 60-s delay trial during the third stage of behavioral testing
Luciferin injection and image acquisition demonstration.
Accelerated snowmelt time-lapse during 2016-2017 snow season
Procedure for the fruit injection as stated in Step 7
Scraping
Dissection procedure
Retinal injury in zebrafish
Video of the partial optic nerve transection procedure.
Image processing
Video of slide preparation
Analysis of clustered HC loss using the main6.m program
Olfactory Habituation Training and Testing
Showing experimental protocol for the establishment of empathy for pain model in laboratory animals.
Electroporation procedure
Precision technique for mouse splenectomy.
Video of dissection procedure
Main steps of sample and slide preparation
Quantification of binding using ImageJ and Excel
Main steps of microscope slide preparation
Harvest peritoneal macrophages
Caesarean sectioning and cross-fostering of mouse